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紫外分光光度法测定氯柳酊中氯霉素和水杨酸的含量
Determination of chloramphenicol and salicylic acidin Lvliu tincture by ultraviolet spectrophotometry
【摘要】 目的 :建立氯柳酊中氯霉素和水杨酸含量测定方法。方法 :采用紫外分光光度法 ,利用吸收度的加和性 ,不经分离直接进行含量测定。结果 :氯霉素的测定波长为 2 78nm ,平均回收率为 10 0 .75 % (RSD=0 .70 % ,n =5 ) ;水杨酸的测定波长为 2 30 nm,平均回收率为 99.4 8% (RSD=0 .2 9% ,n =5 )。结论 :本法简便、快速 ,结果准确。
【Abstract】 bject: To establish a method for the determination of chloramphenicol and salicylic acid in Lvliu tincture. Methods: According to the addition of abs orbance, the contents of two constituents were directly determined without separ ation by ultraviolet spectrophotometry. Results: The determination wavelengths w ere 278 nm and 230 nm for chloramphenicol and salicylic acid, and their average re coveries were 100.75%(RSD 0.70%, n=5) and 99.48%(RSD 0.29%, n=5), respec tively. Conclusion: This method was simple, rapid and accurate.
【Key words】 ultraviolet spectrophotometry; chloramphenicol; sali cylic acid;
- 【文献出处】 天津药学 ,Tianjin Pharmacy , 编辑部邮箱 ,2004年06期
- 【分类号】R927.2
- 【被引频次】1
- 【下载频次】90