【摘要】 目的 :测定肝移植患者术后乙肝免疫球蛋白 ( HBIG)滴度 ,根据所测 HBIG滴度指导临床用药。方法 :微粒子酶免疫法 ( MEIA )测定 HBIG血清谷浓度。结果 :应用 MEIA 测定 HBIG滴度日内精密度为 3.36 %± 1.37% ,日间精密度为 4 .2 0 %± 0 .6 2 % ,回收率为 10 5 .33%± 1.85 %。当给予肝移植患者 HBIG、拉米夫定、他克莫司 ( FK5 0 6 )等药品时 ,HBIG的血清谷浓度与剂量之间存在正相相关。为有效预防术后乙肝复发 ,术后 7d HBIG滴度 >5 0 0 IU/L,8～ 90 d>2 5 0 IU/L,以后保持在 10 0 IU/L以上。结论 :MEIA 法测定 HBIG滴度快速、准确。对于肝移植患者 ,监测 HBIG的血清谷浓度可有效地指导临床给药 ,预防乙肝复发更多还原

【Abstract】 Object: To determine the concentration of hepatitis B immunoglobulin in liver transplantation recipients and provide a guide for clinical drug use. Methods: Patients after liver transplantation were administrated HBIG. The trough serum concentrations of HBIG were determined by MEIA II. Results: The precision ofintra day and inter day were 3 36%±1.37% and 4.20%±0.62%, respectively. The average recovery of HBIG was 105.33%±1.85%. The combination of HBIG and lamivudine may be prophylaxis therapy for preventing the HBV reinfection after liver transplantation. Conclusion: The method of MEIAⅡis simple, convenient and accurate. It can provide a guide for clinical drug use and may prevent HBV reinfection after liver transplantation by determining the trough serum concentrations of HBIG.更多还原