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小鼠胚胎干细胞培养相关影响因素的研究
Studies Related to Factors Affecting the Growth of Mouse Embryonic Stem Cells
【摘要】 本实验主要探讨饲养层、白血病抑制因子(LIF)、水、血清对小鼠胚胎干细胞培养的影响。结果表明:饲养层细胞在小鼠胚胎干细胞的分化抑制过程中有一定作用,而起主导作用的是外源白血病抑制因子;经亚沸处理的MilliQ水,能显著提高小鼠胚胎干细胞保持未分化状态的能力;血清的品质对小鼠胚胎干细胞的培养效果影响显著(p<0.05)。
【Abstract】 Factors affecting in vitro growth of mouse embryonic stem cells were i nvestigated in this study. Feeder layer has certain action in suppressing ES cel l differentiation in culture, while exogenous LIF play predominant role in main taining ES cells undifferentiation. Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS can improve the ability of ES cells t o maintan their undifferentaion state in vitro.
【基金】 国家863计划资助项目(2002AA206605)
- 【文献出处】 动物科学与动物医学 ,Animal Science and Veterinary Medicine , 编辑部邮箱 ,2004年09期
- 【分类号】Q813
- 【被引频次】1
- 【下载频次】308