【摘要】 <正>记忆缺失性贝类毒素(amnesicshellfishtoxin,简称.ASP)主要成分软骨藻酸(domoicacid,简称DA)是一种氨基酸类的生理活性物质(图1),因最早从红藻属的树枝软骨藻(chondriaarmata)分离出来而被命名为软骨藻酸。自1987年加拿大首次发生集体贝类食品中毒事件后,人们从赤潮藻类中的硅藻属(diatom)的多列尖刺菱形藻(nitzschpungensf.multiseries)中检测到了DA,随后,美国、加拿大、北欧一些国家,澳大利亚、日本等国先后从紫贻贝(myltilusedulis)、扇贝(pectenmaximus)、文蛤(callistachione)等贝类体内以及鲭鱼和石蟹的内脏中检测到了DA。鱼、贝通过滤食毒藻,将DA富积在体内,人类因食用被DA污染的鱼、贝而中毒,中毒症状包括恶心、呕吐、腹痛、腹泻等,同时有晕眩、更多还原

【Abstract】 A rapid, selective and sensitive LC/ESI - MSn method was developed for the quantitative determination of domoic acid in shellfish. Samples were extracted with methanol - water followed by clean-up of the extracts with strong anion exchange solid phase extraction cartridge(SPE 3 mL LC - SAX) . A reversed phase HPLC using a mobile phase of acetonitrile - water - trifluoroacatic acid( 13:86.9:0.1 % by volume) and an electrospray ionization(ESI) ion-trap mass spectromrter were used for assay. Data of domoic acid for LC/ESI - MS2 were used for quantitation. Data of domoic acid for LC/ESI - MS3 were used for confirmation. The detection limit was ca. 0.2 μg/g.更多还原