【摘要】 <正>银杏叶制剂作为治疗和预防动脉粥样硬化的有效药物,越来越为人们关注。银杏叶成分复杂,其中银杏黄酮具有扩张血管,降血脂,改善微循环的作用;银杏内酯是血小板活化因子强拮抗剂,对免疫系统,中枢神经系统,缺血损伤有保护作用,并有抗休克、抗过敏及抗炎作用。由于银杏内酯含量较低,对其进行的药动力学研究较少,且多采用的是非特异性的生物测定方法。我们参照中国药典有关药物制剂生物利用度和生物等效性试验的指导原则和相关实验方法,建立的GC-MS-SIM方法,可准确测定血浆中银杏内酯A(GA)和银杏内酯B(GB)的含量,为其药动力学的研究提供了可靠的分析手段。更多还原

【Abstract】 In order to study the bioavilability of ginkgolides, a method for determination of Ginkgolides A and Ginkgolides B in dog plasma was established by GC - MS - SIM. The detection limits of GA and GB were 10 μg·L-1. The recovery for extraction of Ginkgolides A and Ginkgolides B was over 90% ; with-day RSD and inter-day RSD were less than 6% . This method was found to be high sensitive. It can be used for pharmacokinetic studies of extracts from leaves of Ginkgo biloba L.更多还原