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脑供氧颗粒剂中淫羊藿苷的RP-HPLC法测定

Determination of Icariin in Naogongyang Granule by RP-HPLC

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【作者】 于敏焦连庆

【Author】 YU Min,JIAO Lian-qing (Academy of Traditional Chinese Medicine and Clinic of Jilin Province.Changchun,Jilin 130021)

【机构】 吉林省中医中药研究院吉林省中医中药研究院 吉林长春130021吉林长春130021

【摘要】 目的:建立脑供氧颗粒剂中淫羊藿苷的含量测定方法。方法:应用RP-HPLC法测定,选用C18色谱柱,流动相为甲醇-水-冰醋酸(35∶25∶0.3),流速1.0mL/min,检测波长270nm,柱温为25℃。结果:线性关系良好,平均加样回收率为98.84%,RSD为1.83%(n=5)。结论:该方法简便准确,可用于脑供氧颗粒剂的质量控制。

【Abstract】 Objective:A determination method of Icariin in Naogongyang Granule was established. Method: The RP-HPLC was carried out on C18 column using methanol-water-acetic acid (35∶25∶0.3)as a mobile phase,the detecting wavelength was set at 270nm,the flow rate was 1.0mL·min-1,the column temperature was at 25℃. Result: The linear relationship was good,the average recovery was 98.84% and RSD was 1.83%( n =5). Conclusion: The method is simple,reliable and suitable for the quality control of Naogongyang Granule.

【关键词】 脑供氧颗粒剂淫羊藿苷RP-HPLC法
【Key words】 Naogongyang granuleIcarinRP-HPLC
  • 【文献出处】 特产研究 ,Special Wild Economic Animal and Plant Research , 编辑部邮箱 ,2004年01期
  • 【分类号】R286.0
  • 【下载频次】20
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