This article discusses about the growth of Pleurotus tuber-regium hyphsa and the formation of fruit-body and sclerotia. The result indicated that the optimum temperature of Pleurotus tuber-regium hyphsa growth was range from 28℃ to 30℃,the optimum carbon source were fructose and sucrose. The Pleurotus tuber-regium hyphsa could have a well use of many organic nitrogen sources,but have a low utility of inorganic nitrogen sources. The optimum pH is 6,and content of water is 60% of the compost.The formation tem...