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利用酵母双杂交体系筛选拟南芥ABA信号新元件
Screening of the new ABA signaling components in Arabidopsis using yeast two-hybrid system
【摘要】 酵母双杂交体系是用活体研究蛋白质间相互作用的有效途径.利用这一体系,从拟南芥的一cDNA表达文库中筛选出了与同源框蛋白AtHB6有相互作用的两蛋白质AtHB7和AtGluRS,提示这两个蛋白有可能是脱落酸(ABA)信号传导途径中的新元件.
【Abstract】 Two-hybrid system has been proven to be one of the highly efficient tools for detecting protein-to-protein interactions in vivo. By using this system two proteins (AtHB7 and AtGluRS) were screened as the interaction partners of AtHB6 from an Arabidopsis cDNA expression library. It indicates that two new ABA signal components were identified.
【关键词】 酵母双杂交体系;
拟南芥;
脱落酸;
AtHB6;
AtHB7;
AtGluRS;
【Key words】 two-hybrid system; Arabidopsis; abscisic acid; AtHB6; AtHB7; AtGluRS;
【Key words】 two-hybrid system; Arabidopsis; abscisic acid; AtHB6; AtHB7; AtGluRS;
【基金】 德国DFG基金资助项目
- 【文献出处】 深圳大学学报 ,Shenzhen University Journal , 编辑部邮箱 ,2004年03期
- 【分类号】Q933
- 【被引频次】3
- 【下载频次】328