【摘要】 目的:探讨整合素β3和纤维粘连蛋白(fibronectin,FN)在人正常及妊娠输卵管黏膜内的表达与变化。方法:应用免疫组化和图像分析技术检测整合素β3及FN在人正常和妊娠输卵管黏膜内的表达。结果:整合素β3在人正常输卵管黏膜上皮纤毛细胞的胞浆内表达。峡部,排卵后显著高于排卵前(P<0.001);壶腹部,排卵后显著下降(P<0.001);伞部,排卵后的4 d内升高,显著高于其它各时期(P<0.001)。输卵管近妊娠部位黏膜上皮纤毛细胞和分泌细胞胞浆内均表达整合素β3,与分泌期正常输卵管黏膜上皮细胞内的表达比差异无统计学意义。正常输卵管黏膜上皮基底膜和基质表达FN,增生期高于分泌期(P<0.001);FN在近妊娠部位输卵管黏膜内的表达量高于分泌期正常输卵管(P<0.001)。结论:输卵管各节段黏膜整合素β3和FN的表达水平依月经周期不同时期而有明显变化。整合素β3可能不参与输卵管妊娠的发生,FN有可能是输卵管内滞留胚胎植入的分子基础之一。更多还原

【Abstract】 Objective:To investigate the expression and change of the β3 integrin subunit and fibronectin (FN) in normal human oviductal tissue during various phases of the menstrual cycle and tubal ectopic pregnant tissue. Methods:Samples of normal (n=29) and pregnant fallopian tube (n=22) tissuses were obtained from women who had normal menstrual cycle and had history of normal pregnancy. Normal oviductal tissues samples were divided into 4 groups based on their menstrual cycle. Both expression and distribution of the β3 subunit and FN were determined with the immunohistochemical method and the image analysis. Results: The β3 subunit was expressed in the cytoplasm of ciliated cells. The expression level of the β3 subunit was higher after ovulation than that before ovulation in isthmus epithelium (P<0. 001), and declined significantly after ovulation in ampullae epithelium(P<0. 001). In umbrella epithelium within 4 days after ovulation, the expression level of the β3 subunit was observed at rather higher level among other phases (p< 0. 001). The ciliated and secretory cells of the epithelium except where the pregnancy occurred in tubal pregnancy expressed the bβ3 subunit, and no significant relationship was found between the normal tissue of the secretory phase and tubal ectopic pregnant tissue(P>0. 05). FN was expressed in the basement membrane of human oviductal epithelium and matrix. The expression level of FN was higher in hyperplastic phase than that in the secretory one(P<0. 001). And it was lower in normal tubal tissue of the secretory phase than that in tubal ectopic pregnant tissue(P<0. 001). Conclusion:The β3 integrin subunit was expressed in the ciliated cells of human oviductal epithelium, and FN was expressed in the basement membrane of human oviductal epithelium and matrix. Their expression and change in oviductal tissue is based on different phases of menstrual cycle. The β3 subunit was possibly not related to the occurrence of tubal ectopic pregnancy. FN could be the potential molecular basis for the tubal ectopic pregnancy.更多还原