【摘要】 目的 :探讨前列腺素 (PG )E1对血吸虫病家兔肝脏纤维化形成过程中内源性干扰素 (IFN)γ表达的影响及其意义。方法 :血吸虫尾蚴皮肤敷贴法感染 14只家兔构建肝纤维化模型。其中 7只家兔于感染后 60d开始静脉应用PGE12 5 μg·kg-1·d-1至 12 0d。观察虫卵肉芽肿数量和面积 ,原位杂交方法检测IFN γ表达 ,苦味酸天狼星红检测胶原纤维总量。结果 :血吸虫病家兔肝纤维化形成过程中胶原纤维含量增加 ( 71 66± 13 5 9) ;外源性PGE1可以明显降低肝脏胶原水平 ( 13 3 8± 4 2 4) ,与模型组比较 ,P <0 0 1。IFN γ积分光密度由模型组 0 10 5±0 0 2 4升高到治疗组 0 43 8± 0 0 76(P <0 0 1)。结论 :PGE1可以通过上调IFN γ表达 ,有效地降低血吸虫病家兔肝脏胶原纤维生成。更多还原

【Abstract】 Objective To investigate the effect of prostaglandin E 1 (PGE1) on expression of endogenous interferon gamma (IFN-γ) under the process of liver fibrosis induced by schistosomia japonicun in rabbits. Methods The model of liver fibrosis, which was percutaneuosly induced by the infection of cercaria of S. japonicum, was set-up in fourteen rabbits, and then seven of them started to be intravenously injected by PGE 1 (2.5 μg·kg -1 ·d -1 ) at 60 d after the infection for 60 d. The expressions of IFN-γ and accumulation of collagen were examined by in situ hybridization and picrosirius, respectively. Results Increased content of collagen was detected in rabbits with schistosomiasis (71.66±13.59 vs 7.92±3.66), and reversed by PGE 1 therapy (13.38±4.24 vs 71.66±13.59, P<0.01). The O.D. values of IFN-γ (0.438±0.076 vs 0.105±0.024, P<0.01) increased significantly after the PGE 1 therapy. Conclusion PGE 1 can effectively protect liver from deposition of collagen and fibrosis via the highly up-regulated expression of endogenous IFN-γ.更多还原