【摘要】 目的 :研究乙型肝炎病毒标志物定量检测结果与 HBV- DNA含量的临床关系。方法 :采用时间分辨荧光免疫定量检测 (TRFIA)和荧光定量 - PCR技术 (FQ- PCR)对 341例血清标本的乙型肝炎标志物 (HBV- M)和 HBV-DNA含量进行检测。结果 :在 16 8例 HBs Ag/ HBe Ag/ Hbc Ab( ) ,10 1例 HBs Ag/ HBe Ab/ HBc Ab( ) ,72例 HBs Ag/HBc Ab( )阳性的三个组别中 HBV- DNA的平均含量分别为 7.4 0、4 .4 5、5 .0 2。把 HBs Ag和 HBe Ag按浓度的不同分为高中低三组 ,其 HBV- DNA的含量分别为 7.12 ,5 .2 3,4 .0 3,7.74 ,6 .72 ,5 .0 5。结论 :HBV- DNA的含量与 HBs Ag和HBe Ag的浓度存在一定的相关性 ,综合分析乙型肝炎标志物和 HBV- DNA的含量对疾病的诊断、治疗以及疗效的观察有较大的指导意义更多还原

【Abstract】 Objective To investigate the relationshi ps between HBV infected patients immunological marker and HBV-DNA concentratio ns.Methods TRFIA and FQ-PCR methods were applied in the analysis of HBV immunological marker and HBV-DNA concentrations 341clinical serum sample s.Results In 168 HBsAg(+)/HBeAg(+)/HBcAb(+) samples(Ⅰ),In 101 HBsAg(+)/HBeAb(+)/HBcAb(+) samples(Ⅱ),In 72 HBsAg(+)/HBcAb(+) samples(Ⅲ), the HBV-DNA average was 7.40、4.45、5.02 .Divide HBsAg and HBeAg into three low growps of high school according to the difference of the density , the content of HBV-DNA ave rage was 7.12、5.23、4.03、7.74、6.72、5.05.Conclusions The resul ts suggest that the mean quantity of HBV-DNA was correlated with HBsAg and HBeA g. Analyses the content of HBV-M and HBV-DNA synthetically, diagnosis,treament and curative effect in the disease observe greater directive significance.更多还原