【摘要】 目的 :了解 p6 3蛋白在鳞癌组织中的表达及其与组织学分级的关系 ,探讨p6 3蛋白在低分化鳞癌鉴别诊断中的应用价值。方法 :采用免疫组织化学EliVision法检测 89例皮肤鳞癌、鼻咽鳞癌、喉咽鳞癌、肺鳞癌、食道鳞癌、宫颈鳞癌、转移性鳞癌 ,2 0例乳腺浸润性导管癌和 10例宫颈正常鳞状上皮石蜡包埋组织的p6 3表达水平 ,并将结果与鳞癌组织学分级进行分析。结果 :89例鳞癌组织和 10例对照组组织中 p6 3蛋白表达全部阳性 ;其中在组织学Ⅰ级组织中p6 3蛋白阳性率分别为 +:5 6 % (19/ 34)、 :4 1% (14 / 34)、 :3% (1/ 34) ,Ⅱ级分别为 +:2 7% (4 / 15 )、 :6 0 % (9/15 )、 :13% (2 / 15 ) ,Ⅲ级分别为 +:8% (2 / 4 0 )、 :2 5 % (10 / 4 0 )、 :6 8% (2 7/ 4 0 ) ,p6 3蛋白表达与鳞癌组织学分级呈正相关 ,且其关联有高度显著性 (C =0 .4 9,χ2 =4 3.5 6 ,P <0 .0 1)。 2 0例乳腺癌p6 3蛋白表达全部阴性。结论 :p6 3蛋白与鳞癌的组织学分级有关 ,尤其是与低分化鳞癌关系密切 ,其表达水平不仅提示鳞癌组织的恶性程度 ,又可作为鳞状细胞癌较可靠的诊断标记物 ,在低分化鳞癌的鉴别诊断中发挥重要作用更多还原

【Abstract】 Purpose To explore the differential diagnostic value of p63 protein expression in squamous carcinomas based on the association of expression levels with histological gradings. Methods The expression of p63 protein was identified by using Elivisions immunohistochemical essay in the paraffin embed tissue sections of 89 cases of squamous carcinomas of skin, nasopharynx, pharyngolarynx, lung, esophagus, uterine cervix and other metastatic carcinomas respectively,20 cases of breast infiltrating ductal carcinomas, and 10 cases of normal cervix squamous epithelial tissue. The expression levels were then compared with the histological grading of squamous carcinoma tissuges. Results 89 cases of squamous carcinomas and 10 cases of normal cervix squamous epithelial cells were shown positive results only. In histological grading Ⅰgroup, the positive rate +:56 %(19/34),:41 % (14/34),:3 % (1/34).In histological grading Ⅱgroup, the positive rate +:27 % (4/15),:60 %(9/15),:13 %(2/15).In histological grading groupⅢ, the positive rate +:8 % (2/40),:25 %(10/40),:68 %(27/40). Significant correlation of p63 protein expression levels with histological grading was well established(C=0.49,χ 2=43.56,P<0.01). In contrary 20cases of breast carcinoma were show all negative results. Conclusions p63 protein expression level is to be highly associated with the histological grading, particularly with poor differential squamous carcinomas ,which not only indicated the malignancy of squamous carcinomas ,but served as a reliable diagnostic marker of squamous carcinomas, playing an important role in differential diagnosis for poor differential squamous carcinomas.更多还原