【摘要】 目的研究麻叶千里光 (SeneciocannabifoliusLess .)抗菌活性成分。 方法以革兰氏阳性菌金黄色葡萄球菌 (Staphylococcusaureus)、枯草芽孢杆菌 (Bacillussubtilis)和阴性菌大肠杆菌 (Es cherichiacoli)为受试菌 ,采用体外抑菌圈法追踪抗菌活性部分 ,利用各种色谱技术进行分离纯化 ,根据理化性质和波谱学数据进行结构鉴定。结果麻叶千里光水提醇沉物的乙酸乙酯萃取部分显示最强的抑菌活性 ,从该部分分离得到 9个化合物 ,均为酚酸类化合物 ,分别鉴定为 :对 羟基苯乙酸 (p hydroxybenzeneaceticacid ,E 1)、对 二苯酚 (1,4 benzenediol,E 2 )、对 羟基苯乙酸甲酯 (methylp hydroxybenzeneacetate ,E 3)、2 ,5 二羟基苯甲酸 (2 ,5 dihydroxybenzoicacid ,E 4 )、2 ,5 二羟基苯乙酸 (2 ,5 dihydroxybenzeneaceticacid ,E 5 )、2 ,5 二羟基苯乙酸甲酯 (methyl 2 ,5 dihydroxybenze neacetate ,E 6 )、3 羟基 4 甲氧基苯甲酸 (3 hydroxy 4 methoxybenzoicacid ,E 7)、对 羟基苯甲酸 (p hydroxybenzoicacid ,E 8)、邻 羟基苯甲酸 (o hydroxybenzoicacid ,E 9)。结论麻叶千里光对金黄色葡萄球菌和枯草芽孢杆菌抑菌效果明显 ,对大肠杆菌作用较弱。其中E 3～E 9为该植物首次分离得到更多还原

【Abstract】 Objective To study the antibacterial constituents of Senecio cannabifolius Less.Methods All the extractants were tested with Staphylococcus aureus,Bacillus subtilis (gram-positive bacilli) and Escherichia coli (gram-negative bacillus),which guided directively the isolation of the antibacterial constituents in vitro.Chemical constituents were isolated by chromatography.Physico-chemical characters and spectroscopic analyses were employed for the structural identification.Results The ethyl acetate fraction partitioned with water from the water extract and the ethanol precipitate of S.cannabifolius Less.showed the significant bacteriostatic activity.Nine compounds were isolated,and the structures were elucidated by chemical and spectroscopic methods as follows:p-hydroxy benzeneacetic acid (E-1),1,4-benzenediol (E-2),methyl p-hydroxy benzeneacetate (E-3),3,4-dihydroxy benzoic acid (E-4),2,5-dihydroxy benzeneacetic acid (E-5),2,5-dihydroxy benzeneacetic acid methyl ester (E-6),3-hydroxy-4-methoxy benzoic acid (E-7),p-hydroxy benzoic acid (E-8),and o-hydroxy benzoic acid (E-9).The extract of S.cannabifolius Less.exhibited potent antibacterial activity against S.aureus and B.subtilis.Among these compounds,E-3～E-9 were isolated from S.cannabifolius Less.for the first time.更多还原