【摘要】 目的 探讨小鼠髓系DC(CD8α-)中4-1BB的功能性表达。方法 采用免疫荧光标记和流式细胞仪检测DC上4-1BB分子的表达,用酶联免疫方法检测DC分泌的IL-2和IL-12。结果 小鼠髓系DC前体细胞中已有4-1BB分子表达,且随着DC的分化发育和成熟,其表达上调。激发型抗4-1BB分子的抗体2A刺激的DC分泌IL-2和IL-12能力增强,而2A抗体联合TNF-α刺激的DC分泌IL-2和IL-12的量明显高于两者单独刺激组(P<0.05)。结论 小鼠髓系DC表达的4-1BB具有功能性。更多还原

【Abstract】 Objective To investigate 4-1BB expressions on mouse myeloid DC (CD8α-). Methods FCM was used to analyze the expression of 4-1BB on mouse myeloid DC and ELISA to determine the concentration of IL-2 and IL-12 in supernatant of DC. Results 4-1BB expressed on myeloid derived precursors and was up regulated during the process of DC maturation. Furthermore, DC stimulated by agonistic mAb to 4-1BB (2A) had efficient ability to secret IL-2 and IL-12. Moreover, DC stimulated by 2A together with TNF-α have more ffficient ability to secret IL-2 and IL-12. Conclusion Mouse myeloid DC expresses functional 4-IBB molecule.更多还原