【摘要】 目的探讨肠道铁调节蛋白信使核糖核酸(IRP2 mRNA)的表达及其对肠道铁代谢的调节作用 方法 选择健康成年Wistar大鼠40只,随机分为对照组(10只)和缺铁组(30只),建立缺铁动物模型 根据血清铁(SI)、sFn、血红蛋白(Hb)测定结果进行分组 火焰法测定SI,放射免疫分析法测定sFn。参照组织/细胞总RNA提取试剂盒提取十二指肠黏膜总RNA逆转录聚合酶链反应(RT-PCR)法检测不同贫血状态下十二指肠中IRI2 mRNA表达、结果 IRP2 mRNA表达:对照组为0.38±0.12,隐性缺铁组为0.39±0.16,轻度缺铁性贫血组为0.51±0.08,中度缺铁性贫血组为0.51±0.14隐性缺铁组与对照组比较无差异(P>0.05);轻度缺铁性贫血组和中度缺铁性贫血组与对照组比较均有显著差异(P均<0.05);轻度缺铁性贫血组与隐性缺铁组及中度缺铁性贫血组比较均无差异(P均>0.05);中度缺铁性贫血组与隐性缺铁组比较有显著差异(P均<0.05)随缺铁程度增加,IRP2 mRNA表达总体上呈上升趋势结论 IRP2是体内铁代谢的重要调节蛋白更多还原

【Abstract】 Objective To explore the expression of IRP2 mRNA and it’s regulating function in intestinal iron metabolism Methods Forty healthy adult Wistar rats were randomly divided into control group (n=10)and iron deficiency group(n=30).The model rats with iron deficiency were established. Serum iron(SI) was measured by flame assay and sFu was measured by radioimmunoassay.The total RNA of the duodenum membrane of rat was extracted hy the kit test.The expressions of IRP2 mRNA in duodenum in different anemia were tested by RT-PCR.Results The expression of IRP2 mRNA in control group was 0 .38 ± 0 .12 ,the group of recessive iron deficiency was 0.39±0.16, the group of mild tron deficiency anemia was 0.51±0. 08 , the group of moderate iron deficiency anemia was 0 .5 ± 0 .14.There was no difference between recessive iron deficiency group and control group(P>0.05).The expres-sions of IRP2 mRNA in mild iron deficiency anemia group and modetate iron deficiency anemia group were significantly higher than those of control group(P<0.05).In both recessive iron deficieney gronp and moderate iron deficiency anemia group, there was no dif-ference with mild iron deficiency anemia group(P>0. 05). There was significant difference between recessive iron deficiency and mod-erate iron deficiency anemia group(P<0.05).The expression of IRP2 mRNA increased as iron deficieney inereased.Conclusion IRP2 is an important regulatory protein in iron metabohsm.更多还原