【摘要】 目的　了解缺铁对大鼠肠道黏膜铁调节蛋白 2和转铁蛋白受体 (TfR)mRNA表达的影响。方法　建立大鼠缺铁动物模型。根据血清铁 (SI)、血清铁蛋白 (SF)、Hb结果分隐性、轻度、中度贫血 3个实验组 ,设正常对照组。取各组缺铁大鼠小块十二指肠黏膜组织 ,研磨后常规提取RNA备用。按照 5′端相同 ,3′端互补的原则 ,根据NCBIGeneBank中IRPs 2和TfR蛋白受体的cDNA序列设计引物 ,以 β actin为内参对照 ,做RT PCR。结果　随着缺铁程度加重 ,十二指肠黏膜中IRP2mRNA表达增加。中、轻度贫血组均高于对照组 (P <0 .0 1) ,中度贫血组高于隐性贫血组 (P <0 .0 5 ) ,有显著性差异 ,而相邻两组间比较无差异 (P >0 .0 5 ) ;TfRmRNA表达与IRP2mRNA有相似现象 ,随着缺铁加重表达增加 ,实验组均高于对照组 (P <0 .0 5 ) ,各试验组之间比较均有差异 (P <0 .0 5 )。IRP2mRNA和TfRmRNA具有显著正相关 (r=0 .80 2　P <0 .0 1)。结论　IRP2在转录后水平通过调控TfRmRNA表达影响肠道铁的吸收 ,是体内铁代谢的重要调节者更多还原

【Abstract】 Objective To study the influence of iron deficiency on the expression of iron regulatory protein (IRP2) mRNA and transferrin receptor (TfR)mRNA in rat intestine.Methods Create animal model of rat with nutritional iron deficiency. According to the measurement of SI,sFn and Hb,the rats for experiments were divided into 4 groups:control group(n=10),iron deficiency group(n=10),mild iron deficiency anemia group(n=10) and moderate iron deficiency anemia group(n=10). A patch of duodenum mucosa of rats with iron deficiency from every group was taken and ground,and then RNA was routinely extracted from these tissues for experiment. According to the cDNA sequences of IRP2 and TfR in NCBI Gene Bank,the primers were designed and β-actin was used for internal competitive reference standard,and then RT-PCR was performed.Results With the iron deficiency increasing,the expression of IRP2 mRNA in the rats′duodenum mucosa elevated. The expression of IRP2 mRNA in both the moderate and mild anemia group are significantly more than that of the control group(P<0.01), but there is no significant difference between the two neighbour groups (P>0.05). Similar to IRP2 mRNA,the expression of TfR mRNA was enhanced when iron deficiency increased.The experimental groups were all significantly higher than the control group(P<0.05).There was significant difference between each two groups(P<0.05). There was positively significant correlation between the expressions of IRP2 mRNA and TfR mRNA (r=0.802 P<0.01) in the same group.Conclusions We suggests that IRP2 serves as an important regulator of iron metabolism in the human body by controlling the expression of TfR mRNA at the post-transcriptional level to regulate iron uptake from the intetine.更多还原