【摘要】 拮抗细菌R26菌株的代谢产物对番茄灰霉病菌等多种植物病原真菌有较好的抑制作用。本试验研究结果表明 ,在含麦芽糖20g,牛肉浸膏8g,酵母浸膏5g的1000mL液体培养基中 ,最有利于其抗菌物质的产生 ;当温度为32℃ ,pH值为7,在250mL三角瓶中装20mL液体培养基 ,接种量为30%(v/v)时 ,R26菌株产生的抗菌物质抑菌活性最高。培养液中抗菌物质活性随着培养时间的延长而增加 ,培养36h达到最高更多还原

【Abstract】 Antagonistic bacteria R26strain,which was isolated from soil by the authors,can produce antibiotic substances being strongly antagonistic to various plant pathogenic fungi,such as Botrytis cinerea etc.This experiment was conducted to study the opti-mum culture conditions of R26.The results showed that the suitable culture medium was a liquid medium(maltose20g,beef ex-tract8g,yeast extract5g),the optimum culture conditions were32℃,pH7,20ml/250ml flask of culture volume and30%(v/v)of in-oculated volume.The antibiotic activity increased with R26culture time,the pick appeared at32hours.更多还原