【摘要】 目的　探讨血浆B淋巴细胞刺激因子 (BlyS)在自身免疫性疾病发病机制中的作用。 方法　选自 2 0 0 2- 0 8～ 2 0 0 3- 0 8的深圳市南山人民医院及美国南加州大学医学院病人 ,80例系统性红斑狼疮 (SLE)、38例类风湿关节性关节炎 (RA)及 2 8例对照。分别用ELISA、特定蛋白仪和RT -PCR法测定血浆BlyS、免疫球蛋白、抗双链DNA和细胞内BlySmRNA的表达。结果　SLE和RA患者血浆BlyS质量浓度明显高于正常组 (P <0 0 1和P <0 0 5 )。BlyS明显升高的SLE患者 ,其血浆IgG和IgA质量浓度也相应升高 ,二者有一定的相关性。且血浆中抗双链DNA抗体阳性率及BlySmRNA的表达均明显高于血浆低BlyS组。 结论　BlyS促进SLE和RA病情的发生。Ig、抗双链DNA抗体产生及细胞内mRNA表达的增加与BlyS的升高有关。更多还原

【Abstract】 Objective To determine whether plasma levels of B lymphocyte stimulator(BlyS) are elevated in patients with autoimmune diseases and correlate with plasma immunoglobulin,anti-dsDNA and BlyS mRNA.Methods Plasma and cells from 80 patients with systemic lupus erythematosus,38patients with rheumatoid arthritis and 28 normal controls were assayed for BlyS,Ig,anti-dsDNA and BlyS mRNA by a antigen-capture enzyme-linked immunosorbent assay(ELISA), Beckman Arry 360 and RT-PCR.Results Plasma BlyS levels were elevated in patients with SLE and RA(P<0.01 and P<0.05).Plasma IgG and IgA levels were elevated in patients with elevated BlyS and correlated significantly with plasma BlyS levels.Plasma anti-dsDNA and BlyS mRNA levels were elevated in SLE with elevated BLyS.Conclusion BlyS may be an important factor in causing polyclonal hepergammaglobulinemia and elevate anti-dsDNA in patients with SLE and RA.Expression of BlyS mRNA is elevated in patients with elevated BlyS levels.更多还原