【摘要】 目的 :探讨抑癌基因 p1 6对胰腺癌 JF30 5细胞的抑制作用。方法 :以L ipofectamine TM2 0 0 0脂质体介导 p1 6基因转染胰腺癌 JF30 5细胞 ,MTT法测定 OD值 ,计算细胞生长抑制率 ,描绘 p1 6基因转染后 JF30 5细胞增殖曲线。结果 :p1 6基因转染对JF30 5细胞具有明显抑制作用 ( t=4.0 6,P<0 .0 1 ) ,细胞抑制率为 2 6.85 % ,细胞增殖曲线较 JF30 5细胞明显降低。结论 :p1 6基因转染对胰腺癌 JF30 5细胞具有明显抑制作用。更多还原

【Abstract】 Objective:To investigate the suppressive effects of p16 gene on the growth of pancreatic carcinoma cell line JF305. Methods:LipofectamineTM2000 liposome mediated p16 gene was transfected into pancreatic carcinoma cells JF305. MTT was used to detect the optical density value of the transfected cells and the growth curve of these cells was obtained. Results:The growth of p16 gene transfected cells was significantly inhibited than that of control group. The suppression rate of pancreatic carcinoma cell line JF305 with p16 transfection was 26.85%. The growth curve of this group was singnificantly lower than that of control group. Conclusion:The growth of pancreatic carcinoma cell line JF305 can be significantly suppressed by p16 gene transfection.更多还原