【摘要】 目的　利用双波长紫外分光光度法测定注射用哌拉西林钠 舒巴坦钠 (1∶1)中哌拉西林的含量。方法　采用双波长分光光度法。选择λ1 =2 0 6nm为测定波长 ,λ2 =2 5 8nm为参比波长 ,以 pH =10的缓冲液为溶剂。 结果　哌拉西林在 0 .0 0～2 8.0 0 μg ml范围内浓度与吸光度呈线性关系 ,A =0 .0 4 198C +0 .0 0 97,r=0 .9994 ,平均回收率为 (95 .2 3± 1.1) % ,RSD =2 3% (n =9)。结论　此法操作快速、简便、准确 ,无需分离 ,成本低 ,不使用有机溶剂 ,在存在舒巴坦干扰的情况下即可测定药物中哌拉西林的含量。更多还原

【Abstract】 Objective To establish a double wavelength ultraviolet spectrophotometry method for determining the piperacillin in compound injection. Methods The wavelengths of 206 nm and 258 nm were chosen to be the main and the basic respectively to measure the content of piperacillin. Results The linear range for piperacillin was 0.00～28.00 μg/ml, A=0.041 98 C+0.009 7, r=0.999 4 (n=5). The average recovery for piperacipllin was (95.23±1.1) %. RSD=2.3% (n=9). Conclusion This method is simple, convenient and accurate.It can be used for quality control of the preparation for injection even though there is disturbance.更多还原