【摘要】 目的　研究肾舒胶囊对血管紧张素Ⅱ (AngⅡ )刺激的大鼠系膜细胞 (MC)增殖及转化生长因子 - β1 (TGF β1 )、Ⅳ型胶原 (Col Ⅳ )mRNA表达的影响。方法　采用血清药理学方法 ,制备肾舒胶囊含药血清 ,用苯那普利作为阳性对照药 ;将肾舒胶囊含药血清加入AngⅡ刺激的大鼠MC中 ,MTT法测定MC增殖情况 ,半定量RT PCR检测TGF β1 、Col Ⅳ的mRNA表达。结果　肾舒胶囊含药血清可明显抑制AngⅡ诱导的大鼠MC增殖及TGF β1 、Col ⅣmRNA表达 ,其作用效果与苯那普利相当。结论　肾舒胶囊可能通过抑制MC增殖及分泌TGF β1 等生长因子 ,减少细胞外基质积聚 ,防治肾小球硬化。更多还原

【Abstract】 Objective To investigate the effect of Shenshu capsule on cell proliferation and mRNA expression of transforming growth factor-β 1( TGF-β 1) and types Ⅳ collagen (Col-Ⅳ) in cultured mesangial cell (MC). Methods Cultured rat MCs were stimulated by angiotensin Ⅱ (Ang Ⅱ). Serum of rats containing Shenshu capsule or benazepril (an angiotensin-convertion enzyme inhibitor) was applied to culture medium. MTT incorporation was used to assess the proliferation of MCs. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of TGF-β 1 and Col-Ⅳ. Results Shenshu capsule serum inhibited cell proliferation of MCs in a time-dependent manner. Significant down-regulation of TGF-β 1 and Col-Ⅳ mRNA were observed in Ang Ⅱ-induced MCs pretreated with Shenshu capsule serum. There was no significant difference between Shenshu capsule treated group and benazepril treated group. Conclusion Shenshu capsule decreases extracellular matrix accumulation by inhibiting MC proliferation and TGF-β 1 secretion. That may be one of its mechanisms for the treatment of chronic renal failure.更多还原