【摘要】 目的　探讨肾舒胶囊对 5 / 6肾切除大鼠残余肾脏的保护作用及机制。方法　制作大鼠 5 / 6肾切除模型 ,1周后随机分为模型组、肾舒胶囊治疗组、苯那普利治疗组 ,同时作假手术对照。肾舒胶囊治疗组及苯那普利治疗组分别给予肾舒胶囊及苯那普利灌胃。术后 12周检测大鼠体重、血压、2 4h尿蛋白定量、肾功能 ,观察肾脏组织病理学改变 ,检测肾组织中血小板源性生长因子 (PDGF)、转化生长因子 β1(TGF β1)、Ⅳ型胶原 (Col Ⅳ )mRNA表达。结果　肾舒胶囊能显著降低 5 / 6肾切除大鼠 2 4h尿蛋白量 ,改善肾功能 ,抑制PDGF、TGF β1、Col ⅣmRNA表达的上调 ,肾脏组织病理显示它能明显抑制残余肾脏的代偿性肥大 ,减轻肾小球硬化及肾间质纤维化 ,其效果和苯那普利相当。结论　肾舒胶囊能延缓 5 / 6肾切除大鼠残余肾脏的病变进展。其作用机制与抑制系膜细胞增殖及细胞外基质积聚有关。更多还原

【Abstract】 Objective To investigate protective effect and mechanism of Shenshu capsule on the remnant kidney of 5/6 nephrectomized rats. Methods Male Wistar rats were subjected to 5/6 nephrectomy and randomly divided into 3 groups: receiving distilled water, Shenshu capsule 1.2 g/(kg·d) or benazepril:an angiotensin-convertion enzyme inhibitor, 6 mg/(kg·d) from day 7 to week 12 after surgery. Age-matched sham-operated rats served as normal controls. At week 12, rats’ body weight, systolic blood pressure and 24 h urinary protein excretion was measured. Blood sample was obtained for plasma creatinine (SCr) and blood urea nitrogen (BUN) determination.Then, rats were sacrificed and left kidney was harvested for further analysis. The change of renal morphology were measured by periodic acid-Schiff (PAS) staining. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of renal mitogenic and profibrogenic genes, including platelet-derived growth factor (PDGF), transforming growth factor-β 1( TGF-β 1) and types Ⅳ collagen (Col-Ⅳ). Results After subtotal nephrectomy, rats developed progressively elevated proteinuria and plasma creatinine, glomerulosclerosis, interstitial inflammation and fibrosis, all of which were abated by Shenshu capsule and benazepril. Shenshu capsule and benazepril reduced the up-regulation of PDGF, TGF-β 1 and collagen types Ⅳ in remnant kidney. Conclusion Shenshu capsule can retard the chronic renal disease through its effects against mesangial cell proliferation and extracellular matrix accumulation.更多还原