【摘要】 对山西临猗地区东亚飞蝗雌雄共64个个体的酯酶动力学特征及体外抑制进行比较研究.结果表明:雌雄个体的非变性聚丙烯酰胺凝胶电泳图谱不存在明显差异,蛋白含量雌性个体为雄性个体的1.04倍,但是以α-NA,β-NA和α-NB为底物,雌性个体的酯酶活性分别是雄性个体的1.40,1.30和1.39倍.体外抑制表明,雌雄个体的B型酯酶含量分别为78.81%和59.70%.更多还原

【Abstract】 General esterases of the oriental migratory locust between the males and females from Linyi,Shanxi were characterized and compared.Between them,no significant difference was found in banding patterns of general esterases when α-naphthyl acetate was used as substrate in nondenaturing polyacrylamide gel electrophoresis.However,enzyme kinetics suggested that esterase activity of the females was respectively,1.40-fold,1.30-fold,and 1.39-fold higher than that of the males when α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate were used as substrates. Inhibition studies showed that most of esterases of the females and males were B-type, being about 78.82 % and 59.70 %,respectively.更多还原