【摘要】 外植体的褐变是组织培养过程中的主要障碍。目前一般认为影响褐变的因素主要有外植体材料、培养基和培养条件。为了防止褐变的发生,主要可以从外植体和培养条件、进行细胞筛选和材料的预处理、抑制剂或者吸附剂的加入等方面入手。酚、酚酶的区域性分布假说、自由基伤害假说、保护酶系统假说是褐变机理方面较为成熟的3种假说。更多还原

【Abstract】 Explant browning is the main obstacle in plant tissue culture.At present,the primary factors causing explant browning are explant materials,pH value,the culture conditions,the ingredients,the states and the ragidities of culture medium.There are some methods preventing explant browning such as perfect explant and culture conditions,cell filtration and materials pretreatment,adding inhibitors or sorbents to culture medium and so on.There are three hypotheses about the mechanism of explant browning:phenol and phenolic-enzyme regional distribution hypothesis,free-radical damage hy-pothesis and protective enzyme system hypothesis.更多还原