【摘要】 构建携带EB病毒特异性增强子oriP不同结构域的CAT报告质粒,以探讨EB病毒特异性增强子与EB病毒核抗原-1结合后的转录增强作用。聚合酶链反应扩增得到增强子oriP全序列,借助于pUC19质粒将其不同结构域克隆到pCAT3Promoter(pCAT3/P)载体。扩增获得oriP全序列;构建得到携带EB病毒特异性增强子Orip不同结构域的CAT报告质粒pCAT3/P-oriP、pCAT3/P-FR、pCAT3/P-DS、pCAT3/P-(FR+DS)等,质粒中目的基因的插入方向经鉴定正确。更多还原

【Abstract】 To construct CAT reporter plasmids with various domains of Orip and to discuss Epstein-Barr virus specificity enhancer’s role of enhancing the transcription after binding to Epstein-Barr virus nuclear antigen-1.The full enhancer se-quence of Orip was obtained with PCR and its various domains were cloned to pCAT3Promoter vector through pUC19plasmid respectively.The full sequence of Orip was obtained;plasmids such as pCAT3/P-Orip?pCAT3/P-FR?pCAT3/P-DS and pCAT3/P-(FR+DS)were constructed and the direction of inserted purpose genes were identified correctly.CAT re-porter plasmids with various domains of Orip are constructed successfully.更多还原