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离子注入L-乳酸产生菌诱变选育研究

Selective Breeding of Microbial Strain with High Yield of L-lactic Acid by Ion Implantation

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【作者】 赵鑫赵良启刘春卉谢红

【Author】 ZHAO Xin~1,ZHAO Liang-qi~1,LIU Chun-hui~2,XIE Hong~(2) (1.Institute of Biology and Technology,Shanxi University,Taiyuan 030006; 2.Shanxi Institute of Biology,Taiyuan 030006,P.R.China)

【机构】 山西大学生物技术研究所山西省生物研究所山西省生物研究所 山西太原030006山西太原030006山西太原030006

【摘要】 采用离子注入技术对一株产L -乳酸的干酪乳杆菌L110Z进行诱变处理。结果表明 ,在离子注入剂量为 1× 10 14 N+ /cm2 时 ,诱变效果较好 ,从正变菌株中反复筛选 ,得到了一株产酸量高的菌株L110Z5 ,产酸量达到了 92g/L ,比出发菌株产酸提高了 18.0 % ,经过连续传代试验 ,其遗传性状稳定 ,表明L110Z5是一株极具工业化前景的高产菌

【Abstract】 A study was made on Lactobacillus casei L110Z with the production of L-lactic acid by the technology of ion implantation.The result showed that the optimal mutagenic effect was at the ion dose of 1×1014 N+ per cm2.After lots of screening tests,a mutant strain was obtained named L110Z5 whose activity of L-lactic acid about 18.0% higher than that of the original strain L110Z.And it was found that the yield of L-lactic acid by the strain L110Z5 was rather stable through successive transfer of cultures.It showed that the strain L110Z5 with higher yield of L-lactic acid had good prospect in industrial application.

【关键词】 离子注入L-乳酸干酪乳杆菌
【Key words】 Ion implantationL-lactic acidLactobacillus casei
  • 【分类号】Q933
  • 【被引频次】17
  • 【下载频次】166
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