【摘要】 天冬氨酸转氨酶是转氨反应的高效催化剂 ,并且对细胞中氮和碳的代谢起到非常重要的作用。上世纪 90年代中期以来 ,从结构的水平上来分析E coli天冬氨酸转氨酶的催化机理 ,以指导酶的改造研究已经成为国外的研究热点。从酶的催化机理、序列及结构特点、活性中心残基组成以及基因多态性对结构和功能的影响四个方面 ,综述近年来有关E coli天冬氨酸转氨酶的研究现状。更多还原

【Abstract】 Aspartate aminotransferases is an effective catalys t for the aminotransformation, and it plays an important role in the metabolism o f carbon and nitrogen in cellsIt has become one of the research hotspots inter nal and external that study on the catalytic mechanism based on the molecular st ructure of the enzyme has guided the improvement of enzymes since the midst of 9 0’s last centaryAchievements related to the catalytic mechanism, the character of the sequence and the structure feature, residues composed of the active site and the effect of Genetic polymorphism on the structure and function of the E coli AspAT were reviewed in this paper更多还原