【摘要】 目的 :研究在轻度修饰低密度脂蛋白 (mm LDL)诱导下差异表达的基因 ,探讨动脉粥样硬化发生的分子机制。方法 :用DDRT PCR(differential displayreversetranscription PCR)技术分析mm LDL诱导下人血管内皮细胞的基因表达差异 ,并用反向Northern证实差异显示基因。结果 :mm LDL诱导下人血管内皮细胞出现一些上调和下调的基因 ,上调基因有胸腺素β4、FGFRI原癌基因伴随蛋白、FK5 0 6结合蛋白、rTSβ蛋白和细胞间粘附分子 1 ;下调基因有Apobec 1结合蛋白 1、细胞色素B5 61和ERP72。结论 :体外mm LDL可诱导HUVEC细胞一些基因表达水平变化 ,它们与血管内皮细胞发生病理变化和动脉粥样硬化斑块形成直接相关更多还原

【Abstract】 Objective: To study the differences of genes’ expression in vascular endothelial cells induced by mildly modified low density lipoprotein (mm-LDL) and to help understand the molecular mechanism of atherosclerosis. Methods: Differential-display reverse transcription-PCR (DDRT-PCR) was used to analyse the differences of genes’ expression in vascular endothelial cells induced by mm-LDL. The differential expressed cDNA was confirmed by reverse Northern blot. Results: The cDNAs of up-regulated and down-regulated genes were isolated in HUVEC induced by mm-LDL. The up-regulated genes included human thymosin β4, FGFRI oncogene partner, FK506 binding protein, rTSβ protein and intercellular adhesion molecular-1 were identified. The down-regulated genes included human Apo becl binding protein 1, cytochrome B561 and ERP72 were identified. Conclutions: Mm-LDL could change the expression of some genes in endothelial cells and result in the pathological changes. Finally, the formation of atherosclerosis speckle could be related to these genes.;更多还原