【摘要】 采用体内急性全身性毒性实验、溶血实验、MTT实验 (Tetrazolium- based colorim etric assay)和材料浸渍液培养细胞的形态学观察法实验等对 Ti- Fe- Mo- Mn- Nb- Zr系合金的生物安全性进行了初步的评价。采用 x射线光电子能谱仪 (XPS)分析了合金表面氧化膜成分 ,并用原子发射光谱仪 (ICP)检测了浸渍液中合金元素的种类和浓度。结果显示 :Ti- Fe- Mo- Mn- Nb- Zr系合金表面形成了以 Ti O2 为主的致密氧化膜 ,浸渍液中均存在浓度为0 .2～ 1.0 7mg/ l的 Fe和 0 .16～ 0 .5 mg/ l的 Mn,这种义齿材料有利于补充人体所需的 Fe和 Mn;未见任何急性毒性反应 ;溶血程度为 0 .5 5 8%～ 0 .6 4 2 % ,有良好的血液相容性 ;细胞毒性实验评价级别为 0和 1级 ,无明显的细胞毒性作用 ;倒置相差显微镜观察细胞形态 ,未发现异常。由以上结果可以初步认为 Ti- Fe- Mo- Mn- Nb- Zr系合金是一种理想的生物材料更多还原

【Abstract】 In this study, the general toxicity tests including acute toxicity test,haemolysis test, MTT assay of Ti Fe Mo Mn Nb Zr alloys were carried out. The morphology of these cells was also observed under phase contrast microscope. By using X ray photoelectron spectroscopy(XPS), the kind and mol% of element in surface film were studied. The kind and concentration of element in dipping fluid were investigated by ICP atomic emission spectrometry. The results showed the primary component is TiO 2 in surface film. The dipping fluid of Ti Fe Mo Mn Nb Zr alloys contains Fe 0 2~1 07mg/l and Mn 0 16~0 5 mg/l;such dental materials are beneficial to health. No cytotoxic effect was disclosed by in vitro and in vivo tests. The level of cytotoxicity was grade 0 and 1; the haemolysis degree was 0.558%~0.642%, i.e. less than 5%. The cells growing in the extract showed normal morphology. These data indicate that Ti Fe Mo Mn Nb Zr alloy, as a dental material,has good biocompatibility.更多还原