【摘要】 通过对繁殖季节黄鳝为孵卵所吐的泡沫的特性和生化成分分析 ,孵卵泡的黏度为 2 72mPaS ,证明了该孵卵泡是糖蛋白 ,其中糖类含量为 0 15 6mg/mL ,蛋白质含量为 0 2 5 0mg/mL ,蛋白质 /糖类比值为 1 6 0。分离的泡沫蛋白经SDS PAGE电泳含 4种组分 ,分子量从小到大依次为 4 2 5kDa、5 8 6kDa、6 5 3kDa、98 2kDa。泡沫蛋白含 16种氨基酸 ,氨基酸总含量为 2 0 1 88mg/ 10 0mL。同时对孵卵泡的生理作用进行了分析和讨论更多还原

【Abstract】 Biochemical compositions and physiological functions of froth that Monopterus albus spits in breeding seasons were reported in this paper.The results showed that the viscosity of the froth was 2.72 mPaS,the froth was glycoprotein,and the content of sugar was 0.156 mg/mL,that of protein was 0.250mg/mL.The ratio of protein to sugar was 1.60. The froth protein was composed of four compositions by SDS-PAGE.The molecular weight of the froth protein was 42.5kDa,58.6kDa,65.3kDa,and 98.2kDa,respectively. It consisted of 16 kinds of amino acids,the total contents of amino acids was 201.88 mg/100mL.At the same time,physiological functions of the froth were analysed and discussed.The physiological functions of the froth included three aspects.Firstly,the hatching rate of oosperm was 76.0%,96.7%,94.2%,and 64.0%at different water temperatures(22℃,25℃,28℃,and 31℃)in the froth groups,respectively,and the hatching rate of oosperm was 22.8%,33.9%,30.2%,and 14.6%in the control groups,respectively.Secondly,the froth could decrease the water mold growth,and prevented oosperm from infecting the water mold.The frequency of the oosperm infected by water mold was 0.0%,2.1%,8.4%,and 12.5% at different water temperatures in the froth groups,respectively,and the frequency of the oosperm infected by water mold was 44.8%,65.4%,82.0%,and 90.5% in the control groups,respectively.The average frequency of the oosperm infected by the water mold was 5.75% in the froth groups,the average frequency of the oosperm infected by the water mold 70.83%in the control groups.Finally,the froth could increase oxygen,and accelerate velum of oosperm cracking.Comparison with effect of different hatching methods on the hatching rate and survival rate of the ricefield eel.The hatching and survival rate of the ricefield eel from high to low was found by the froth incubation>the dripping water incubation>the sleeping water incubation.更多还原