【摘要】 建立了用高效液相色谱法测定蜜桶花颗粒及其原植物来江藤中麦角甾苷含量的方法。实验采用C18柱,以甲醇 0 5%醋酸水(体积比为42∶58)为流动相,在334nm检测波长处检测。结果表明:麦角甾苷的进样量为0 029～2 038μg时,进样量与色谱峰面积有良好的线性关系(r=0 9998);回收率为90 2%～93 8%;方法的精密度好,相对标准偏差(RSD)为0 34%(n=5)。方法快速、简便、准确,所测结果稳定、重现性好,可作为蜜桶花颗粒质量控制的一个检测方法。更多还原

【Abstract】 A high performance liquid chromatographic method was established for the determination of acteoside in Mitonghua particles and Brandisia hancei. The results showed that acteoside can be extracted more efficiently with butanol than with water. Therefore, both Mitonghua particles and Brandisia hancei were extracted with butanol. The extracts were analysed on an RP-18 column using a mixture of CH₃OH-0.5% aqueous HAc （42∶58, v/v） as the mobile phase with UV detection at 334 nm. A good linear response was observed within the range of 0.029-2.038 μg of analytes （r=（0.999 8））. The recoveries were 90.2%-93.8% and the relative standard deviation was 0.34% （n=5）. This rapid, simple, accurate, and reproducible method is proposed for the quality control of Mitonghua particles.更多还原