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氨基酸的分析方法及其应用进展

Development of Analytical Methods for Amino Acids and Their Applications

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【作者】 丁永胜牟世芬

【Author】 DING Yongsheng~1, MOU Shifen~2 (1. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China; 2. Research Center for Eco-environmental Sciences, The Chinese Academy of Sciences, Beijing 100085, China)

【机构】 北京大学药学院化学生物系中国科学院生态环境研究中心 北京100083北京100085

【摘要】 从衍生试剂角度,介绍了不同衍生化氨基酸的分析方法,包括离子交换色谱法、高效液相色谱法、气相色谱法和毛细管电泳法,以及无需衍生化的直接分析法高效阴离子交换色谱 积分脉冲安培法,并总结了蛋白质、食品和生理体液样品中的氨基酸分析方法。

【Abstract】 Different analytical methods for amino acids, including ion exchange chromatography (IEC), high performance liquid chromatography (HPLC), gas chromatography (GC) and capillary electrophoresis (CE) are introduced in light of derivatization. High performance anionic exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD) method for direct amino acid analysis was also presented. The applications of the methods for amino acid analysis in proteins, foods, physiological fluids and chiral separation as well are also reviewed.

【关键词】 氨基酸分析衍生化应用综述
【Key words】 amino acidanalysisderivatizationapplicationreview
  • 【文献出处】 色谱 ,Chinese Journal of Chromatography , 编辑部邮箱 ,2004年03期
  • 【分类号】O656
  • 【被引频次】170
  • 【下载频次】2987
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