【摘要】 盐酸克伦特罗是一种只具有免疫反应性不具有免疫原性的小分子半抗原。本文采用重氮化法将其与牛血清白蛋白交联,用紫外扫描和SDS变性凝胶电泳方法检验。并用合成的CL_BSA免疫小鼠,经ELISA检验,小鼠产生了针对CL的抗体,表明合成的CL_BSA复合物具有免疫原性,为盐酸克伦特罗单克隆抗体的制备及其检测试剂盒的研制奠定了基础。更多还原

【Abstract】 Clenbuterol is a small molecular hapten that has immunoreactivity but hasn’t immunogenicity. It is coupled with bovine serum albumin by diazotization in this article. And the Clenbuterol_BSA conjugate is identified by ultra violet scan, SDS- PAGE and indirect inhibited ELISA .The results demonstrated that the Clenbuterol_BSA conjugate has immunogenicity which is good for next monoclonal antibody preparation and the research of ELISA kit .更多还原