【摘要】 从番茄果实中提取脂肪氧化酶,确定提取脂肪氧化酶的最适条件,并在此基础上用硫酸铵分步沉淀提纯脂肪氧化酶;结果表明,脂肪氧化酶纯化了3.4倍,活性回收达84%;将获得的高活力的脂肪氧化酶氧化亚油酸制备过氧化物亚油酸;产物用带二极管阵列检测器的反相高效液相色谱法检测分析,该方法简便,分析时间短,很容易跟踪脂肪氧化酶氧化亚油酸产生过氧化氢物的过程。更多还原

【Abstract】 Lipoxygenase was extracted from tomato fruits,and the optimal extraction conditions for lipoxygenase activity were discussed. Then lipoxygenase was purified by ammonium sulphate fractionation. The results showed that lipoxygenase was purified 3.4-fold,and the recovery rate of lipoxygenase activity was 84%. Then 9-hydroperoxides from linoleic acid were prepared by the purified tomato lipoxygenase. The product was detected by reversed-phase HPLC with diode array detector. The results showed that the method was easy to track analysis of hydroperoxides of lipoxygenase products formed from linoleic acid in short time.更多还原