【摘要】 研究了各种营养因子对重组大肠杆菌产β-胡萝卜素的影响,以葡萄糖10g/L和蔗糖10g/L为复合碳源,牛肉膏4g/L为氮源有利于菌体生长和β-胡萝卜素的表达,添加微量因子NaCl、Span-20和KH2PO4有利于β-胡萝卜素的表达,而对细胞生长作用不明显,最佳添加浓度分别为1.6、0.7和3g/L。更多还原

【Abstract】 In this paper, the nutrient factors onβ-carotene production of recombinant Escherichia coli were studied. Compoundcarbon sources, glucose 10g/L and sucrose 10g/L, and beef extract 4 g/L used as nitrogen sources were favor for cell growth andβ-carotene expression. Addition NaCl1.6g/L,Span-20 0.7g/L and KH2PO4 3g/L helped forβ-carotene expression., But hadno effect on cell growth.更多还原