【摘要】 目的:研究玉米须多糖(SMPS)对CCl4肝损伤小鼠的保护作用。方法:取健康的雄性昆明种小鼠(20±2)g50只,按体重随机分成5组,每组10只,即正常组(饲喂正常饲料),CCl4肝损伤组(正常饲料+CCl4),低剂量组(正常饲料+CCl4+0.1mlSMPS溶液/只),中剂量组(正常饲料+CCl4+0.2mlSMPS溶液/只),高剂量组(正常饲料+CCl4+0.4mlSMPS溶液/只)。SMPS溶液提前5d每天灌胃,对照组与肝损伤组灌胃0.4ml生理盐水,末次灌胃后2h给0.10%CCl4 0.4ml致伤。18h后摘除眼球取血,测定血清谷丙转氨酶(SGPT)、谷草转氨酶(SGOT)、乳酸脱氢酶(LDH)活性,破腹取肝脏,测定肝脏丙二醛(MDA)、谷胱甘肽(GSH)含量及肝脏指数,并对肝细胞的组织形态学进行观察。结果:预先灌SMPS溶液,具有显著地抑制CCl4引起的小鼠血清SGPT、SGOT、LDH及肝脏MDA含量、肝脏指数的升高以及肝脏GSH含量的降低,并能显著地减轻CCl4引起的肝小叶内的灶性坏死。结论:玉米须多糖对CCl4致小鼠肝损伤具有保护作用。更多还原

【Abstract】 Objective: To study the protective function of Gardeniayellow(SMPS) against CCl4-induced hepatotoxicity in mice.Methods: Healthy Kunming male mice, weighting (20±2)g, 50 ones were randomized into 5 groups with 10 per-group .control group, CCl4-injured group, low dose group (CCl4 injured+0.1ml SMPSsolution), medium dose group(CCl4-injured+0.2mlSMPS solution) and high dose group(CCl4-injured+0.4mlSMPS solution). SMPS solution was given i.g.5d prior to CCl4 injury.Glutamate-pyruvate transaminase (SGPT), glutamic-oxaloacetic transaminase (SGOT) and lacticde hydrogenase(LDH) activi-ties in serum were determined 18h after CCl4-injury. Hepaticmalondialdehyde (MDA), glutathione (GSH) and liver index werealso detected. Results: In SMPS treated groups, the increases of SGOT, SGPT and LDH activities in serum were inhibitedobviously. The decreases of liver GSH were reversed actually. The elevations of MDA and liver index were prevented significantly.The lesions in liver lobule were ameliorated obviously. Conclusion: Gardeniay ellow canpro-tectagainst CCl4-inducedhepatotoxicity.更多还原