【摘要】 制备了牛血清白蛋白——桔霉素包被抗原和卵蛋白——桔霉素免疫抗原,用卵蛋白——桔霉素连接物免疫小白鼠,间接ELISA测小鼠抗血清效价,待血清效价达到要求后,腹腔注射骨髓瘤细胞,10～15d后抽取腹水离心得抗体。建立竞争ELISA检测红曲样品桔霉素含量的方法,研究了该法的特异性和桔霉素的加标回收,结果表明,此法具有较高的特异性和加标回收能力,桔霉素的测定浓度范围为5.0～200ng/ml,回收率97%～104%。更多还原

【Abstract】 The immune antigen of OV-citrinin and the coated antigen of BSA-citrinin were synthesized respectively andidentified with ultraviolet absorption spectrometry. The competitive ELISA for detecting the citrinin content in the Hongquproduct was developed, with the detecting concentration range 5.0～200ng/ml of citrinin. The recovery of this method is 97%～104%.更多还原