【摘要】 在固定化磷脂酶A1时,利用酶抑制剂与磷脂酶A1活性中心氨基酸残基结合,使戊二醛基与磷脂酶A1的非活性氨基残基缩合,从而起到保护酶活性中心基团,提高固定化酶活力作用。实验证明磷酸盐是磷脂酶A1的竞争性抑制剂,并求出Ki值。更多还原

【Abstract】 When immobilized PLA1, inhibitor can couple amine residues which are important for catalysis or maintaining proteinstructure. Therefore, it can protect amine groups of activating site by binding between glutaradehyde and inactive groups of amineso as to increase activity retention of immobilized enzyme.In addtion, phosphate is shown to be a competive inhibitor of PLA1,and by means of making chart to get the Ki value.更多还原