【摘要】 目的建立测定南果梨中绿原酸含量的高效液相色谱法。方法C18色谱柱(250×4.6mm,5μm);流动相甲醇-0.4%磷酸溶液(2575);检测波长327nm。结果精密度试验RSD为1.9%,n=6;加样回收率98.5%～102.6%,RSD为1.6%～2.1%。结论南果梨果心、果肉、果皮中绿原酸的含量分别为1.785、0.271、0.714mg/g。更多还原

【Abstract】 Objective: To establish a new HPLC method for determination of chlorogenic acid(CGA)in Nanguoli pear.Method:CGA was analysed by using C18 column(250mm×4.6mm,10μm) with detector at 327nm, and the mobile phase consistingof CH3OH-0.4% H3PO4 (25:75).Results: The recoveries of CGA was 98.5%～102.6%,precision (RSD) was 1.6%～2.1% (n=5).Conclusion:Quantitative analysis showed that the content of CGA in the core peel and pulp of Nanguoli pearwere 1.785, 0.271 and 0.714mg/g respectively.更多还原