【摘要】 经氯仿-乙醇分级分离,丙酮沉淀及DE-32纤维素柱层析的方法,从乌骨鸡红细胞中得到纯化的Cu,Zn-SOD。结果表明此酶的比活力为11,954U/mg,提纯倍数为334.71,聚丙烯酰胺凝胶电泳蛋白带与活性带相对应。相对分子量约为32,000,相对亚基分子量约为16,000。最大紫外吸收波长为260nm,对KCN和H2O2敏感,最适pH为6～9,热稳定性在60℃以下。更多还原

【Abstract】 Copper/zinc superoxide dismutase (Cu,Zn-SOD) from black-bone chicken erythrocytes has been purified bychloroform-ethanol fractionation through acetone precipitation and DE-32 chromatography. The results showed that the spe-cific activity of the enzyme was 11,954U/mg , and its purification factor was 334.71. This enzyme showed two identicalsubunits.The molecular weight was about 32,000,and the identical subunit molecular weigh about 16,000.The ultraviolet ab-sorption peak of the enzyme was found at 260nm .The enzyme was sensitive to KCN and H2O2.The enzyme optimum pH wasfrom 6 to 9 and stable below 60℃.更多还原