【摘要】 确定了高效液相色谱法快速测定扁桃种仁中α-VE含量的方法。样品以正己烷为提取溶剂,超声提取30min,最后用无水乙醇溶解定容。以98%甲醇为流动相,在Europher-100-C18(25cm×40mm×5μm)的色谱柱上,柱温35℃,检测波长280nm,流速1.0ml/min,α-VE线性范围为0.2～20mg/L,线性相关系数为0.9997,检出限0.2mg/L,平均回收率97.3%,RSD%=0.92～3.12。将该法用于扁桃仁中VE含量的测定,方法简单、快速,结果准确、可靠。更多还原

【Abstract】 Tocopherol in almond kernel was rapidly determined by HPLC. Sample was prepared through Ultrasonic Waveenhancing extraction of α-Tocopherol for 30min in hexcane. The determination was performed in Europhe. 100-C18 column(25cm×4.0mm×5μm) at 35℃ with a mobile phase of 98% methanol. The flow rate was 1.0ml/min. a-Tocopherol was detectedat 280nm. The range of linearity was from 0.2mg/L to 100mg/L (r=0.9997). The lower limit of detection of α-Tocopherol was0.2mg/L, The average recovery was 92.7%～103% and RSD was 0.3%～l.0%, The results showed that the method is simple andtrustworthy in determination of vitamine E in the almond kernel.更多还原