【摘要】 采用高速逆流色谱法分离纯化大豆异黄酮中的大豆苦和染料木昔。溶剂系统为乙酸乙酯一醋酸一水,体积比为5:1:10,上相为固定相,下相为流动相,逆流色谱仪转速为800r/mm,流速为1.5ml/min。所得大豆昔、染料木昔经高效液相色谱分析测定,纯度分别达到98.2%和99.2%(面积归一法)。更多还原

【Abstract】 Semlpreparation high speed coufltercurent chromatography(HSCCC)was used for the systematic optimization andpreparation of the two-phase solvent system to separate Daidzin and Genistin from soybean isoflavones extracted from soybeanseed.The two-phase solvent system composed of ethyl acetate-acetic acid-water(5:l:10 in volume ratio).The mobile phase is thelower phase nd operated at flow-rate of 1.5ml/min, while the apparatus rotated at 800r/min.High performance liquidchromatographic analysis of Daidzin and Genistin revealed that their purity was individually 98.2%and 99.2%.更多还原