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10L容积光生物反应器批次培养盐藻的初步研究

Studies on the Batch Cultivation of Dunaliella salina Using a Photobioreactor with a Volume of 10 Liter

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【作者】 姜建国朱跃辉黄洋

【Author】 Jiang Jianguo Zhu Yuehui Huang Yang(College of Food and Bioengineering,South China University of Technology,Guangzhou,510640)

【机构】 华南理工大学食品与生物工程学院华南理工大学食品与生物工程学院 广州510640博士副教授广州510640510640

【摘要】 设计和制作了容积为 1 0L的螺旋管式光生物反应器 ,螺旋管光照单元为 1 6个 ,光照比表面积增大为 95m- 1,反应器外观设计为 3层框架式结构 ,缩小了占地面积。用此光生物反应器进行了盐藻的批次培养试验 ,确定批次培养的最佳接种量为OD6 30 =0 1 5 ,最佳藻液循环流量为 63 0mL/h ,并对此光生物反应器批次培养的生长情况和 β 胡萝卜素产量进行了数学曲线拟合 ,生物量近似为一次曲线CX =0 0 91 9+0 0 3 64t;β 胡萝卜素产量近似为二次曲线CX =0 3 848t2 -1 1 693t+1 73 3 8。

【Abstract】 A photobioreator with volume of 10 L were designed and make up. The illumination units helix were 16,which brings to the augment of illuminate surface area to 95m -1 . For reducing floor space,the facade of the reactor was designed as three-layer frame. By doing experiments of batch cultivation with this photobioreator,optimum inoculum volume was determined at OD 630 =0.15 and the optimum circulation rate at 630?mL/h in batch culture. With the help of mathematic tool,the growth of D. salina in batch culture can be simulated as curve equation C x = 0.091 9+0.036 4t, and the output of β-carotene was calculated as C x= 0.384 8t2-1.169 3t+1.733 8.

【基金】 广东省科技攻关项目 (99M0 14 10G)
  • 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2004年11期
  • 【分类号】TQ920
  • 【被引频次】5
  • 【下载频次】280
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