【摘要】 研究了南瓜多糖提取液的分离与纯化。试验结果表明 ,提取液用TCA法脱蛋白 ,去除率达 94.3 3 % ,采用SephacrylS 40 0HR凝胶柱层析逐步分析纯化 ,分离得到 2种多糖PP Ⅰ和PP Ⅱ。利用醋酸纤维膜电泳法对PP Ⅰ和PP Ⅱ进行纯度检测 ,表明 2种组分都达到了均一的纯度。更多还原

【Abstract】 The separation and purification of the polysaccharide from pumpkin was studied in this paper By using TCA method, 94.33% of protein was removed from the extracted juice. Further purification by Sephacryl S-400 HR gel-column produced two types of polysaccharide: PP-I and PP-II. The purity of PP-I and PP-II were examined by acetate fiber film electrophoresis method. The results show that the purity of two compositions are both homogeneous.更多还原