【摘要】 研究了以甘油为底物 ,由克雷伯氏菌 (Klebsiellapneumoniae)生产 1 ,3 丙二醇的流加发酵中底物的不同流加策略 (如甘油反馈连续流加、脉冲流加、恒速流加、甘油 碱耦合流加、改进的甘油 碱耦合流加 )对发酵的影响。研究表明 ,采用改进的甘油 碱耦合流加策略 ,即甘油 碱耦合流加同甘油恒速流加相结合的流加策略 ,能够有效地控制发酵体系中甘油浓度 ,当发酵进行至 5 0h时 ,1 ,3 丙二醇浓度可达 5 4g/L ,生产强度为 1 0 8g/(L·h) ,明显优于其他流加策略下 1 ,3 丙二醇的发酵结果。更多还原

【Abstract】 Different feeding strategies, including continues feeding, pulse feeding,constant rate feeding,coupling feeding and modified coupling feeding of glycerol and base, were studied in 1,3 propanediol fed batch fermentations by Klebsiella pneumoniae with glycerol as substrate. The results indicated that the modified coupling feeding, which combines base and glycerol mixture for pH control with constant rate feeding,could effectively control the glycerol concentration in the fermentation process. This new feeding strategy could give 54?g/L of 1,3 propanediol with a productivity of 1 08?g/ (L·h), which is obviously better than the fermentation results by other feeding strategies.更多还原