【摘要】 目的　研究自由基在骨骼肌缺血再灌注损伤中的作用。方法　用 3 2kg重物挤压家兔双后肢大腿和臀部 4h或 5h制作骨骼肌缺血再灌注损伤的动物模型 ,分别测定实验动物的血浆、组织自由基及细胞损伤的变化。结果　两挤压伤组在解压后 2h内平均动脉压明显低于对照组 (P <0 0 5 ) ,血浆丙二醛 (MDA)、一氧化氮 (NO)含量和N 乙酰 β D 氨基葡萄糖苷酸酶 (NAG)活性在解压后 2 4h均高于对照组 (P <0 0 5或 <0 0 1) ;两挤压伤组比较 :压伤 5h组平均动脉压在解压后 2h内低于压伤 4h组 (P <0 0 5 ) ,而血浆MDA、NO、NAG及组织MDA、NO也高于压伤 4h组 (P <0 0 5 )。结论　骨骼肌缺血时可产生大量的自由基 ,从而造成骨骼肌缺血再灌注损伤更多还原

【Abstract】 Objective To explore the role of free radical played in ischemia-reperfusion injury of skeletal muscles.Method The experimental ischemia-reperfusion injury model was made by crushing the hindlimbs and buttocks of the rabbit with a 32 kg heavy object for 4 h or 5 h (two groups),then measured the activities of SOD,NAG and the levels of MDA,NO in plasma and crushed muscles,respectively.Result The mean arterial blood pressures of the rabbits in both crushing groups were lowered comparing with that of the control (P<0.05) two hours after removing the object,while the NAG activity and the levels of MDA,NO were obviously rised (P<0.01)24 h after removing the object.The levels of MDA,NO in heart,lung,liver,kidney,intestine and skeletal muscle tissues in both crushing groups were significantly higher than those in the control group(P<0.05).BP in crushing 5 h group was lower than that in crushing 4 h group (P<0.05),while the activity of NAG and levels of MDA,NO in plasma and in tissues in crushing 5 h group were higher than those in crushing 4 h group (P<0.05).Conclusion The free radicals were induced by ischemia-reperfusion injury of skeletal muscles and related to time of skeletal muscles ischemia.更多还原