【摘要】 对培养嗜酸乳杆菌的基础培养基进行筛选,确定为11％NFM培养基。采用正交试验对增殖因子进行优化,得到最优增菌培养基:基础培养基+5％番茄汁+10％萝卜汁+0.1％肝浸汁+5％啤酒。以最优增菌培养基测定生长曲线、pH值和滴定酸度的变化,确定增菌培养终止时间为10hr,此时活菌数达7.49×10~9cfu/ml。更多还原

【Abstract】 11% NFM medium was founded by screening several radical media to cultivate Lactobacillus acidophilus. Based on the 11% NFM medium, the optimal medium was obtained by orthogonality test consisted of tomato juice 5%,carrot juice 10%, liver infusion 0. 1%, beer 5%. Growth curve with optimized stimulated culture was determined by following the change of pH and titratable acidity. According to the growth curve. the terminal time was in 10 hours and Lactobacillus acidophilus got to 7. 49×10~9cfu/ml.更多还原