【摘要】 在建立PVYV和ACLSVRT PCR优化体系的基础上,主要研究了多重PCR中2套引物的浓度和退火温度的影响,研究表明:多重PCR体系中引物Tm值越高、长度越长浓度就需越高;如果Tm值相差不大,退火温度的确定要以Tm值高的引物为准。应用该体系可以节约成本、缩短时间。更多还原

【Abstract】 Based on the optimized RT-PCR detection system of two viruses, the paper studies the concentration of primers and annealing temperature in multiple PCR, and establishes multiple PCR system that can detect two viruses simulta neously.The multiple PCR system is economical and timesaving.更多还原