【摘要】 目的考察聚乙二醇 (PEG)修饰对溶菌酶和粒细胞集落刺激因子 (G CSF)活性的影响。方法以不同方法活化的PEG修饰溶菌酶 ,通过正交试验和单因素考察确定合适的修饰条件 ;溶菌酶活力测定采用溶壁小球菌法 ,抗原性测定采用试管沉淀法 ;G CSF活性测定以小鼠血浆中中性粒细胞数目增殖情况反映。结果当偶联上一个PEG分子时 ,溶菌酶的活性保留 13% ,抗原性显著减弱 ;G CSF经PEG修饰后对小鼠中性粒细胞数目的增加有显著促进作用 ,且作用时间明显延长。结论PEG修饰对G CSF血循环半衰期的延长有显著作用更多还原

【Abstract】 PurposeTo investigate the influence of polyethylene glycol(PEG) modification on the activity of lysozyme and granulocyte colony-stimulating factor(G-CSF).MethodsDefining the suitable condition of modification by orthogonal test and single agent test. The activity of lysozyme was tested according to the Pharmacopeia and the antigenicity tested by tube sedimentation. The activity of G-CSF was examined by injecting subcutaneously into normal mice and determining neutrophil counts at various times.Results13% of the modified enzyme activity was maintained while the antigenicity was greatly reduced. The modified G-CSF can maintain elevated neutrophil levels for extended periods.ConclusionPEG modification can elongate apparently the half life of G-CSF.更多还原