【摘要】 采用正硅酸乙酯与N (β 氨乙基 ) 氨丙基三乙氧基硅烷在油包水形成的微胶囊中同步水解的方法 ,一步法制备了氨基化的二氧化硅颗粒 ,得到的颗粒粒径在 0 3～ 0 5μm之间 ,平均大小为 0 3 7μm ,氨基含量和颗粒大小可控 ,氨基含量高达 5 6mmol g。此颗粒经戊二醛处理后 ,采用共价法固定木瓜蛋白酶 ,固定化最适pH6 5,最佳给酶量为 15mg g载体 ,固定化酶的最适反应温度为 70℃ ,最适反应pH为 6 5,固定化酶热稳定性 ,pH耐受性 ,贮存稳定性都明显高于游离酶 ,表明此颗粒可作为一种优良的酶固定化载体更多还原

【Abstract】 Numerous approaches and materials have been explored for preparation o f immobilization enzymes because they have considerable advantages over enzyme i n bulk solution. Among present enzyme carriers inorganic materials have lower co st and more stability than organic materials. But the present inorganic material s contain less active sites and have to undergo some complicated processes befor e binding enzyme. So it is necessary to explore a new kind of inorganic enzyme c arrier. Papain, a well characterized thiol protease kinetically and structurally , is a suitable model to compare the efficiency of various immobilization proced ures. SiO 2 particles containing amine groups were synthesized by synchronous h yd rolysis of tetraethylorthosilicate(TEOS) and N-(2-aminoethyl)-3-aminopropylt rime thoxysilane (AEAPS) in W/O Microemulsion of Triton X-100/cyclohexane/ammonium h yd roxide system. The obtained particles have a diameter range from 0 3μm to 0 5 μm, and their average size is 0 37μm and can be controlled by adjusting water to su rfactant molar ratio and water to TEOS and AEAPS molar ratio. Compared with trad itional porous silica beads, these particles contain much more amine groups and their amine group content can be easily changed in the process of synthesis. Pap ain was immobilized on the particles which were treated by glutaraldehyde with c ovalent method. The optimum immobilization conditions of enzyme were as follows: enzyme load was 15mg/g carrier, pH was 6 5. The pH and temperature optima were 6 5 and 70℃ for immobilized enzyme. All above indicate this kind of particle can be a good enzyme immobilization carrier.更多还原